Faculty of Food Technology and Biotechnology, Department of Chemistry and Biochemistry, Laboratory of Toxicology

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Main research field

Toxicology

Specific research areas

toxicology, toxicity testing, alternative methods, analytical toxicology

Techniques/methods

cell culture techniques, Trypan blue exclusion method, MTT assay, Kenacid blue assay, Neutral red assay, apoptosis/necrosis detection

Equipment

automatic CO2 incubator, laminar flow hood, transmission light microscope, inverted light microscope, temperature controlled centrifuge with fixed-angle and swing-out rotors, UV-VIS spectrophotometer

Technology keywords

  • Clinical Research, Trials
  • In vitro Testing, Trials
  • Toxicology
  • Food quality and safety
  • Food Microbiology/Toxicology/Quality Control

Services offered

  • Teaching and Training
  • Quality control
  • Live organism culture preparation

Market sectors

  • Beverages
  • Food Producers
  • Industrial Engineering
  • Health Care Equipment & Services
  • Pharmaceuticals & Biotechnology

Short summary (English)

The primary research interest in our laboratory is directed towards the events at cellular and molecular level after exposure to certain xenobiotics such as industrially originated pollutants (polychlorinated biphenyls, polycyclic aromatic hydrocarbons, etc.), pesticides (organochlorine insecticides, herbicides ...) or generally persistent organic pollutants which could have potential adverse impact on human health. Also, there are numerous indications that exposure to such chemicals may induce dysfunction of animal and human reproductive system. Therefore, our objectives are better understanding the mechanisms underlying the different biological responses induced by endocrine disrupting compounds using both in vitro and in vivo studies. In the in vivo experiments involving laboratory animals, the evaluation of certain endocrine disrupting effects on gonadotropic system, specially reflecting on spermatogenic process, was conducted. Also, in order to promote the higher animal welfare practice with implementation the „Three Rs“ approach our laboratory focuses its efforts on application of alternative in vitro toxicity tests. The introduction of animal cell culture techniques enable us to determine qualitatively and quantitatively cytotoxic effects and subtile biochemical and morphological changes on cellular level that points out the risk of residual xenobiotic concentrations present in the environment to which members of an ecosystem and humans are exposed.

Short summary (Croatian)

Temeljni interes u istraživanjima našeg laboratorija usmjeren je prema zbivanjima na staničnoj i molekulskoj razini kao posljedica izloženosti određenim ksenobioticima poput zagađivača industrijskog porijekla (poliklorirani bifenili, policiklički aromatski ugljikovodici itd.), pesticide (organoklorirani insekticidi, herbicidi …) ili općenito perzistentni organski zagađivači koji potencijalno mogu štetno utjecati na zdravlje ljudi. Također, brojne su indikacije da izloženost takvim kemikalijama može izazvati disfunkciju reprodukcijskog sustava u životinja i čovjeka. Stoga su primjenom in vivo i in vitro studija naši ciljevi usmjereni boljem razumijevanju mehanizama različitih bioloških odgovora potaknutih djelovanjem endokrinih disruptora. Korištenjem laboratorijskih životinja u eksperimentima in vivo provedene su procjene štetnih učinaka endokrinih disruptora na gonadotropni sustav, posebno onih koji se odražavaju na process spermatogeneze. U cilju promicanja veće dobrobiti za eksperimentalne životinje implementacijom “tri R” pristupa u istraživanjima naš laboratorij je usmjerio nastojanja na primjenu alternativnih in vitro testova toksičnosti. Uvođenje tehnika koje koriste kulture životinjskih stanica omogućuje kvalitativno i kvantitativno određivanje citotoksičnih učinaka, kao i suptilnih biokemijskih i morfoloških promjena na staničnoj razini, koje upozoravaju na rizik od rezidualnih koncentracija ksenobiotika u okolišu kojima su izloženi pripadnici ekosustava, uključujući čovjeka.

Last update: 06.02.2013. 06:08:18

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