Faculty of Science, Division of Chemistry, Department of Analytical Chemistry

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Main research field

Analytical chemistry

Specific research areas

analytical chemistry atomic and molecular spectroscopy (ICP-AES, UV-Vis spectroscopy, spectrofluorimetry, vibrational spectroscopy), HPLC study of molecular structure and interactions analysis of f

Techniques/methods

surface-enhanced Raman spectroscopy (SERS), FT-Raman spectroscopy, FT-IR spectroscopy, attenuated total reflectance, UV-Vis spectrometry, spectrofluorimetry, ICP-AES, HPLC.

Equipment

FT-IR/Raman spectrometer Bruker Equinox 55 equiped with fiber optics, FT-IR spectrometer Bruker Vector 22, CARY 3 UV-Visible Spectrophotometer, Analytic jena SPECORD 200, Perkin Elmer Luminescence Spectrometer LS 50, ICP spectrometer (Prodigy, Teledyne Leeman Labs, Hudson NH, USA), Flame photometer, PFP-7, Buck Scientific, MWS-2 Microwave System Speedware BERGHOF, Hewlett Packard 1090 HPLC with ProStar 330 DAD detector and Varian Auto Smpler 410

Technology keywords

  • Ceramic Materials and Powders
  • Organic Substances
  • Analytical Chemistry
  • Ecology
  • Inorganic Substances, Pharmaceuticals

Services offered

  • Technique/method optimization and testing
  • Material characterisation (testings, measurements, various quantitative and / or qualitative analyses)
  • Preparation of environmental or energy related studies
  • ICT services (molecular modeling, ab initio methods, in silico methods etc.)

Market sectors

  • Alternative Energy
  • Industrial Engineering
  • Support Services
  • Health Care Equipment & Services
  • Pharmaceuticals & Biotechnology
  • Specialty Chemicals

Short summary (English)

Investigations in the Department of analytical chemistry are carried out within several research projects. Surface-enhanced Raman spectroscopy is applied to study interactions and binding modes of biologically active small molecules with nucleic acids as well as to investigate adsorption mechanisms of the studied molecules on the nanoparticles in colloidal suspensions of silver and gold. Methods of vibrational spectroscopy (infrared spectroscopy, Raman spectroscopy) are used to study molecular structures of various organic compounds able to exist in different tautomeric and isomeric forms in aqueous and organic media. In-line Raman spectroscopy is being used for the real-time in situ monitoring of chemical and physical processes, e. g. chemical reactions and crystallization. Because of its advantages over standard off-line methods, in-line Raman spectroscopy has recently been used in pharmaceutical industry as well as in preliminary investigations of biologically active compounds. UV-Vis spectrometry and spectrofluorimetry is used for determination of equilibrium constants (tautomeric, protonation etc.) of different compounds (Schiff bases, hydrazones etc.) in solution. New methods are developed for extraction and/or spectrophotometric and spectrofluorometric determination of analytes based on interactions of metal ions and different commercial or newly synthesized ligands. Inductively coupled plasma atomic emission spectrometry (ICP-AES) is used for the determination of minor and major elements in fruits (e.g. apples, berries) and edible oils. Also, the differentiation of edible oils regarding their geographical origin based on the trace elemental content is done. Different samples (food, pharmaceutical products etc.) are analyzed qualitatively and quantitatively by high performance liquid chromatography (HPLC).

Short summary (Croatian)

Istraživanja u Zavodu za analitičku kemiju provode se u sklopu nekoliko znanstvenih projekata. Primjenom spektroskopije površinski pojačanog Ramanovog raspršenja istražuju se interakcije i načini vezanja biološki aktivnih malih molekula s nukleinskim kiselinama te proučavaju mehanizmi adsorpcije istraživanih molekula na nanočestice u koloidnim suspenzijama srebra i zlata. Metode vibracijske spektroskopije (infracrvena spektroskopija, Ramanova spektroskopija) primjenjuju se pri istraživanju molekulske strukture raznih organskih spojeva koji mogu postojati u različitim tautomernim i izomernim oblicima u vodenom i organskom mediju. Ramanova spektroskopija in-line se koristi za in situ praćenje kemijskih i fizičkih procesa (primjerice kemijskih reakcija i procesa kristalizacije) u realnom vremenu. Zbog svojih prednosti u odnosu na standardne off-line metode, Ramanova spektroskopija in-line se u poslijednje vrijeme koristi u farmaceutskoj industriji, kao i za preliminarna istraživanja biološki aktivnih tvari. UV-Vis spektrometrija i spektrofluorimetrija primjenjuje se za određivanje ravnotežnih konstanti (tautomerne, kiselinsko-bazne itd.) različitih spojeva (Schiffove baze, hidrazoni) u otopini. Razvijaju se nove ekstrakcijske i/ili spektrofotometrijske i spektrofluorimetrijske metode određivanja analita temeljene na interakciji iona metala s različitim komercijalnim ili novo sintetiziranim ligandima. Atomska emisijska spektrometrija uz induktivno spregnutu plazmu (ICP-AES) primjenjuje se za određivanje metala u voću (npr. jabukama i različitom bobičastom voću) i jestivim uljima. Na temelju sastava elemenata u tragovima određuje se geografsko porijeklo jestivih ulja. Različiti uzorci (hrana, farmaceutski proizvodi) se kvalitativno i kvantitativno analiziraju primjenom tekućinske kromatografije visoke djelotvornosti (HPLC).

Last update: 11.02.2013. 05:58:47

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