High recovery purification of viruses by immunoaffinity chromatographyPDF
- Pharmaceuticals & Biotechnology
High purity virus particles are a prerequisite for efficient and safe viral biomedicines. Removal of impurities while preserving delicate macromolecular virus particle structure is a challenging task. Scientists from the University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology developed an efficient elution procedure using native conditions in immunoaffinity chromatography. The novel procedure results in pure virus particles with preserved structural integrity and infectivity.
- Pure viral particles
- Highly concentrated virus particles
- Possibility of scale-up
- Viral vaccines
- Oncolytic virotherapeutics
- Fundamental virus research
We are looking for companies who operate in this market with the view of cooperation in development, intellectual property protection, and bringing the technology to market.
Stage of development
Concept of native elution in immunoaffinity chromatography has been developed at the University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology.
Our scientists achieved up to 70% yield in immunoaffinity chromatography purification of mumps virus and showed that virus suspension has become enriched in infective virus particles, while the contribution of non-infective particles was reduced.
National patent application in Croatia and PCT application filed.
Details can be found here.
An application note from BIA separations is available here.
Title: Nonspecific native elution of proteins and mumps virus inimmunoaffinity chromatography
Authors: Marija Brgles, Dora Sviben, Dubravko Forčić, Beata Halassy
Journal: Journal of Chromatography A
Last update: 07.06.2018. 09:55:23